Gwyntwr1386 Science Journal

A Review of The Role of Hemoglobin in Different types of Cancer ISSN 2753-8176 (online), DOI:10.13140/RG.2.2.24610.20164 Ana Pedro1 1Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK Keywords: 1. Corresponding author: info@gwyntwr1386.com A role for haemoglobin in cancer diagnosis in general is supported by a good number of publications. A previous work indicated the possibility of a direct relationship between the hemoglobin level and not only the early stages of cancer, but also the chemically induced precancerous condition (1). Interestingly, in patients with a diagnosis of primary lung, colorectal, breast or liver cancer, higher post-diagnosis haemoglobin change regardless of the baseline haemoglobin levels and the direction of changes associates with a significantly shorter survival (2). Here we summarize, the results of our findings in what concerns proteomics analyzis of several different cancer patient samples and of cancer cell line...

Therapy with DNA damaging drugs of a small number of non-metastatic and metastatic colorectal cancer patients Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Introduction and results Colorectal cancer (CRC) is the third most common malignancy and the second most deadly cancer, inducing estimated 1.9 million incidence cases and 0.9 million deaths worldwide in 2020 (1). Each year, approximately, 230,000 patients are treated with adjuvant chemotherapy (AT) (2) . Nine patients with non-metastatic (NM) and metastatic (M) CRC were treated with 5-fluorouracil (5-FU)/leucovorin (LV) (LV5FU) regimen (3), 3 patients with M disease with FOLFOX (folinic acid (leucovorin, FOL), fluorouracil (5-FU, F), and oxaliplatin (Eloxatin, OX) (4) and 2 patients with NM and M disease were treated with XELOX regimen (capecitabine (trade name Xeloda) combined with oxaliplatin (5) (tables 1 and 2). All the patients...

Hemoglobin in Acute Leukemias, Brief Report Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Introduction Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells, where there is a rapid growth of abnormal cells that build up in the bone marrow and blood interfering with normal blood cell production (1). Here, we analyze by proteomic analysis the precipitates after ultra-centrifugation of the conditioned media resulting from the cell culture of OCI-AML3 and Kasumi1 cell lines. We compare these proteomic profiles with those derived from the precipitates after ultra-centrifugation of plasma samples of control adult males (Control plasma male 1 and Control plasma male 2) OCI-AML3 cell line is derived from a 57 year-old male with Adult acute myeloid leukemia (2). Kasumi1 cell line is derived from the peripheral blood of a male AML patient, 7 weeks old (3). Resul...

Hemoglobin and Children’s cancers - Brief Report, DOI:10.13140/RG.2.2.21321.52325, ISSN 2753-8176 (online) Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Wilms tumor or nephroblastoma , is a cancer of the kidneys which occurs most commonly as a renal tumor in child patients (1). Neuroblastoma, a rare type of cancer, also mostly affects babies and young children. It develops from neuroblasts which were left behind during baby's development in the womb (2).Most often starts from one of the adrenal glands but can also develop in the neck, chest, abdomen or spine (3). IMR5 is a neuroblastoma cell line, derived from a male, aged 1 year and 1 month, derived from a metastatic site in the abdomen (4). SKNBe2 is another neuroblastoma cell line, derived from a 2 year old male and from bone marrow metastasis after chemotherapy and radiotherapy (5). Finally, CCG9911 is a Wilm’s tumor cell line (6)...

Hemoglobin and osteoblasts markers in bone cancer Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Osteosarcoma is a type of bone sarcoma or primary bone cancer which usually develops in growing bones and can affect any bone although most common areas are the arms or legs. It is most often found in teenagers and young adults, although it can happen at any age. Around 1 in 10 osteosarcomas (10%) develop in adults in their 60s and 70s (1). Ewing tumors, however can start in either the bone or the soft tissue (2). Here, we analyzed by proteomics analysis, the content of the precipitates derived from the ultracentrifugation of conditioned media derived from the cell culture of different bone cancer cell lines (table 1, 3-6). We found out that a set of different hemoglobin subunits and osteoblast markers express differently in these different bone cancer cell lines (Table 2, 7) In summary, while cell...

Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Squamous-cell carcinoma (SCC ), also known as epidermoid carcinoma, comprises a number of different types of cancer which begin in squamous cells (1). Squamous cells are present on the surface of the skin, on the lining of hollow organs in the body, and on the lining of the respiratory and digestive tracts (1). About 90% (2) of cases of head and neck cancer (cancer of the mouth, nasal cavity, nasopharynx, throat and associated structures) are due to SCC. The most common types of head and neck cancers occur in the lip, mouth, and larynx (3). We analyzed by proteomics the conditioned media precipitates of two different samples of head and neck cancer samples culture, M2 and M9 (table 1). We found that hemoglobin beta and hemoglobin delta are consistently found in both samples with significant Mascot scores. Materials and Methods Proteomic analysis was ...

Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com There are two main types of lung cancer , categorized by the size and appearance of the malignant cells, small cell lung cancer (SCLC; 15% of lung cancer diagnoses) and non-small cell lung cancer(NSCLC; 85% of diagnoses) (1). Here we analyze by proteomic analysis, the precipitates derived from conditioned media of different lung cancer cell lines (2-7), all NSCLC and also derived from the culture of a bone metastases from lung cancer sample. In what concerns, adenocarcinomas, HBB (hemoglobin beta) and HBA (hemoglobin alpha) scores raise from primary cancer to lymph node (LN) metastases (Table 1). HBG2 (hemoglobin gamma-2) may appear with a NS (non-significant) score in primary cancer, but becomes significant in LN metastases (mets). In other NSLCs types, HBB score raises from primary cancer to LN metastases. Finally, bone metastases, show a spectacula...