Hemoglobin and osteoblasts markers in bone cancer, ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.14509.05606

Hemoglobin and osteoblasts markers in bone cancer Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Osteosarcoma is a type of bone sarcoma or primary bone cancer which usually develops in growing bones and can affect any bone although most common areas are the arms or legs. It is most often found in teenagers and young adults, although it can happen at any age. Around 1 in 10 osteosarcomas (10%) develop in adults in their 60s and 70s (1). Ewing tumors, however can start in either the bone or the soft tissue (2). Here, we analyzed by proteomics analysis, the content of the precipitates derived from the ultracentrifugation of conditioned media derived from the cell culture of different bone cancer cell lines (table 1, 3-6). We found out that a set of different hemoglobin subunits and osteoblast markers express differently in these different bone cancer cell lines (Table 2, 7) In summary, while cell lines such as SAOS and SKES1 seem not to express osteoblast markers, cell lines such as 143B and U20S, may express these markers. Moreover, while cell lines such as 143B and SKES1 express HBB (hemoglobin subunit beta), HBA (hemoglobin subunit beta alpha) and HBG2 (hemoglobin subunit gamma-2), cell lines such as SAOS, additionally express HBD (hemoglobin subunit delta, P02042) and U2OS does not express HBG2, but expresses a different HBD variant (E9PFT6). Materials and Methods Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017 (8). Only proteins with Mascot scores of approximately 90 or >90 were considered (9) The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at Hemoglobin in Head and Neck cancer - Short report (ISSN 2753-8176 (online) | Zenodo References 1. Osteosarcoma | Macmillan Cancer Support 2. Soft tissue sarcoma | Macmillan Cancer Support 3. SK-ES-1 - HTB-86 | ATCC 4. Saos-2 - HTB-85 | ATCC 5. U-2 OS - HTB-96 | ATCC 6. 143B - CRL-8303 | ATCC 7. PAUTKE et al. (2004). Characterization of Osteosarcoma Cell Lines MG-63, Saos-2 and U-2 OS in Comparison to Human Osteoblasts. ANTICANCER RESEARCH 24: 3743-3748 8. https://www.biorxiv.org/content/10.1101/202291v1 9.

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