Posts

A2M IN ACUTE LEUKEMIA - BRIEF REPORT (ISSN 2753-8176 (online))

Image
A2M IN ACUTE LEUKEMIA - BRIEF REPORT (ISSN 2753-8176 (online)) 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 592 Old Chester Road, Birkenhead, CH42 4NW, info@gwyntwr1386.com Introduction Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells, where there is a rapid growth of abnormal cells that build up in the bone marrow and blood interfering with normal blood cell production (1). Here, we analyze by proteomic analysis the precipitates after ultra-centrifugation of the conditioned media resulting from the cell culture of OCI-AML3 and Kasumi1 cell lines. We compare these proteomic profiles with those derived from the precipitates after ultra-centrifugation of plasma samples of control adult males (Control plasma male 1 and Control plasma male 2) OCI-AML3 cell line is derived from a 57 year-old male with Adult acute myeloid leukemia (2). Kasumi1 cell line is derived from the peripheral blood of a male AML patient, 7 weeks old (3). Results and Discus...
Post a Comment
Read more

A2M IN BONE CANCER (ISSN 2753-8176 (online)) - brief report

Image
A2M IN BONE CANCER (ISSN 2753-8176 (online)) - brief report 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 58 Grove Rd, Birkenhead, CH42 3XX. info@gwyntwr1386.com Osteosarcoma is a type of bone sarcoma or primary bone cancer which usually develops in growing bones and can affect any bone although most common areas are the arms or legs. It is most often found in teenagers and young adults, although it can happen at any age. Around 1 in 10 osteosarcomas (10%) develop in adults in their 60s and 70s (1). Ewing tumors, however can start in either the bone or the soft tissue (2). Here, we analyzed by proteomics analysis, the content of the precipitates derived from the ultracentrifugation of conditioned media derived from the cell culture of different bone cancer cell lines (table 1). We found out that A2M is present in all cancer cell lines types with significant scores and that is present in higher levels in the osteosarcoma cell line 143B rather than in Ewing’s sarcoma cell lin...
Post a Comment
Read more

A2M IN GASTRIC CANCER (ISSN 2753-8176 (online))

Image
A2M IN GASTRIC CANCER (ISSN 2753-8176 (online)) 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 58 Grove Rd, Birkenhead, CH42 3XX. info@gwyntwr1386.com Gastric cancer is a cancer which develops from the lining of the stomach(1). The majority of the cases are gastric carcinomas, which can be divided into a number of subtypes, including gastric adenocarcinoma (2). Unfortunately, early gastric cancer causes few symptoms and the disease is usually advanced when the diagnosis is made being difficult to treat (3). We analyzed by proteomics the secretion of A2M by different gastric cancer cell lines derived from primary tumor tissues (4) or lymph node metastases (5) or stage IV tumors (6) in cell culture. We found out that (table 1), A2M presents significant scores in primary gastric cancer cell lines and in lymph node metastases cell lines, whereas is not present or is present in a non-significant way in gastric cancer stage IV cell lines. This points out a role for A2M in gastric...
Post a Comment
Read more

Can Hemoglobin be used to detect and monitor cancer progression? An update (ISSN 2753-8176 (online))

Can Hemoglobin be used to detect and monitor cancer progression? An update (ISSN 2753-8176 (online)) 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 58 Grove Road, Birkenhead, CH42 XX. info@gwyntwr1386.com The rationale for developing a lab based assay for cancer - Mammography screening is meant to reduce breast cancer mortality through early diagnosis and treatment of disease that would otherwise threaten life. However, in some cases a screening mammogram detects a cancer that would have neither caused death nor presented clinically during the woman’s lifetime. It is clear that to reduce the burden of over diagnosis on both the emotional and physical health of the patient and the economic health of the NHS, more sensitive tests for the diagnosis of breast cancer are needed (1, 1b, 1c). An hemoglobin based test would be very specific, unexpensive and very accessible as for the reasons detailed below. - Total hemoglobin and different hemoglobin subunits seem to be assoc...
Post a Comment
Read more

A social Cafe to tackle drug addiction (ISSN 2753-8176 (online))

A social Cafe to tackle drug addiction (ISSN 2753-8176 (online)) 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 58 Grove Road, Birkenhead, CH42 XX. info@gwyntwr1386.com Description of the innovation To set up Netherlands bulldog like cafes at the NHS where you can freely consume cannabis, cocaine, methamphetamide, etc for free and moreover you can have a free delicious meal and enrolling different healthy activities. What problem is your innovation trying to solve? Drug addiction What diseases or conditions does your innovation impact? Mental health and behavioural conditions - Drug misuse
Post a Comment
Read more

A2M in Prostate Cancer – short report (ISSN 2753-8176 (online))

Image
A2M in Prostate Cancer – short report (ISSN 2753-8176 (online)) 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 58 Grove Road, Birkenhead, CH42 XX. info@gwyntwr1386.com Alpha-2-macroglobulin (A2M) is a large protein found in the blood which is mainly produced by the liver. A2M is the largest major non-immunoglobulin protein in human plasma. We study the possibility that A2M can play a role in prostate cancer (PC) progression. Prostate cancer is the second most common type of cancer in the world. Moreover, is the fifth leading cause of cancer-related mortality among men (1). About 99% of cases occur after age 50 (1). Early prostate cancer symptoms are often similar to those of benign prostatic hyperplasia and include frequent urination, nocturia (increased urination at night), difficulty starting and maintaining a steady stream of urine, hematuria (blood in the urine), dysuria (painful urination) as well as fatigue due to anemia, and bone pain (1). Like early cancer, meta...
Post a Comment
Read more

A2M in Lung Cancer – short report (ISSN 2753-8176 (online))

Image
A2M in Lung Cancer – short report (ISSN 2753-8176 (online)) 1.Ana Pedro 1.Gwyntwr1386 Health & Social Care, 58 Grove Road, Birkenhead, CH42 XX. info@gwyntwr1386.com Alpha-2-macroglobulin (A2M) is a large protein found in the blood which is mainly produced by the liver. A2M is the largest major non-immunoglobulin protein in human plasma. Alpha-2-macroglobulin (A2M) is a large protein found in the blood which is mainly produced by the liver. A2M is the largest major non-immunoglobulin protein in human plasma. We study the possibility that A2M can play a role in lung cancer initiation and/or progression. Here we analyse by proteomic analysis, the presence of A2M (P010230) in the precipitates derived from conditioned media of different lung cancer cell lines and lung cancer-derived bone metastases (mets) (table 1). We find out that control cell WI38 presents quite a big Mascot score (993), while primary lung cancer cell lines present an average Mascot score level (315-415)...
Post a Comment
Read more