Hemoglobin in Lung Cancer - Short report (ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.30186.59842

Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com There are two main types of lung cancer, categorized by the size and appearance of the malignant cells, small cell lung cancer (SCLC; 15% of lung cancer diagnoses) and non-small cell lung cancer(NSCLC; 85% of diagnoses) (1). Here we analyze by proteomic analysis, the precipitates derived from conditioned media of different lung cancer cell lines (2-7), all NSCLC and also derived from the culture of a bone metastases from lung cancer sample. In what concerns, adenocarcinomas, HBB (hemoglobin beta) and HBA (hemoglobin alpha) scores raise from primary cancer to lymph node (LN) metastases (Table 1). HBG2 (hemoglobin gamma-2) may appear with a NS (non-significant) score in primary cancer, but becomes significant in LN metastases (mets). In other NSLCs types, HBB score raises from primary cancer to LN metastases. Finally, bone metastases, show a spectacular raise on HBB and HBA, appears HBD (hemoglobin delta) with a very strong score, also HBG1 (hemoglobin gamma-1) and HBE (hemoglobin epsilon), and HBZ (hemoglobin zeta) is non-significant (table 1). Materials and Methods Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017 (8). Only proteins with Mascot scores of approximately 90 or >90 were considered (9) The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at Hemoglobin in Prostate Cancer | Zenodo References 1. Tanoue, Mazzone & Tanner 2022, "Evidence for Lung Cancer Screening". 2. NCI-H292 [H292] - CRL-1848 | ATCC 3. NCI-H1650 [H-1650, H1650] - CRL-5883 | ATCC 4. ECACC General Cell Collection: 90071810 PC-9 (formerly known as PC-14) (culturecollections.org.uk) , Cellosaurus cell line PC-9 (CVCL_B260) 5. NCI-H358 [H-358, H358] - CRL-5807 | ATCC 6. NCI-H1975 [H-1975, H1975] - CRL-5908 | ATCC 7. NCI-H2228 [H2228] - CRL-5935 | ATCC 8. https://www.biorxiv.org/content/10.1101/202291v1 9.

https://f1000research.com/articles/7-283

Amendments:

Please, see https://zenodo.org/record/8130817 for additional files. 1118 - lung cancer cell line, WI38 - 3 month old female embryo lung tissue cell line

1118 - hemoglobin gamma-2 (E9PBW4), hemoblobin beta (P68871),and hemoglobin alpha (P69905) with Mascot Scores of 153.20, 277.89 and 526.39, respectively.WI38 -hemoblobin beta (P68871),and hemoglobin alpha (P69905) with Mascot Scores of 348.33 and 969.56, respectively.