A2M IN ACUTE LEUKEMIA - BRIEF REPORT (ISSN 2753-8176 (online))

A2M IN ACUTE LEUKEMIA - BRIEF REPORT (ISSN 2753-8176 (online))

1.Ana Pedro

1.Gwyntwr1386 Health & Social Care, 592 Old Chester Road, Birkenhead, CH42 4NW, info@gwyntwr1386.com

Introduction

Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells, where there is a rapid growth of abnormal cells that build up in the bone marrow and blood interfering with normal blood cell production (1). Here, we analyze by proteomic analysis the precipitates after ultra-centrifugation of the conditioned media resulting from the cell culture of OCI-AML3 and Kasumi1 cell lines. We compare these proteomic profiles with those derived from the precipitates after ultra-centrifugation of plasma samples of control adult males (Control plasma male 1 and Control plasma male 2) OCI-AML3 cell line is derived from a 57 year-old male with Adult acute myeloid leukemia (2). Kasumi1 cell line is derived from the peripheral blood of a male AML patient, 7 weeks old (3).

Results and Discussion

We found out that (table 1) Mascot scores are high in the Kasumi1 and OCI-AML3 samples and extremely high both in the control plasma male 1 and control plasma male 2 suggesting that A2M may play a role in preventing the development of AML.

Material and Methods

Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017 (4). Only proteins with Mascot scores of approximately 90 or >90 were considered (5) The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at https://zenodo.org/record/8108731

References

1."Adult Acute Myeloid Leukemia Treatment". National Cancer Institute. 6 March 2017. Retrieved 19 December 2017.

2.https://www.cellosaurus.org/CVCL_1844

3.https://www.atcc.org/products/crl-2724

4. https://www.biorxiv.org/content/10.1101/202291v1

5.https://f1000research.com/articles/7-283

6. https://gwyntwrscience.blogspot.com/2023/07/hemoglobin-in-acute-leukemias-brief.html

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