A2M in Prostate Cancer – short report (ISSN 2753-8176 (online))

A2M in Prostate Cancer – short report (ISSN 2753-8176 (online))

1.Ana Pedro

1.Gwyntwr1386 Health & Social Care, 58 Grove Road, Birkenhead, CH42 XX. info@gwyntwr1386.com

Alpha-2-macroglobulin (A2M) is a large protein found in the blood which is mainly produced by the liver. A2M is the largest major non-immunoglobulin protein in human plasma.

We study the possibility that A2M can play a role in prostate cancer (PC) progression.

Prostate cancer is the second most common type of cancer in the world. Moreover, is the fifth leading cause of cancer-related mortality among men (1). About 99% of cases occur after age 50 (1). Early prostate cancer symptoms are often similar to those of benign prostatic hyperplasia and include frequent urination, nocturia (increased urination at night), difficulty starting and maintaining a steady stream of urine, hematuria (blood in the urine), dysuria (painful urination) as well as fatigue due to anemia, and bone pain (1). Like early cancer, metastic prostate cancer often causes bone pain, often in the vertebrae, pelvis, or ribs (1).

Here we analyse by proteomic analysis, the presence of A2M (P010230) in the precipitates derived from conditioned media of different prostate cancer cell lines (table 1). We find out that lymph node (LN) derived cell line LNCap presents the highest Mascot score, 724.15, followed by Vcap (cell line derived from vertebral metastases), 449.53. Both these cells lines present androgen sensitivity and are positive for androgen receptor (AR).Morever, cell lines PC3 (derived from vertebral metastases) and DU145 (derived from brain metastases) and both not sensitive to androgen and negative to AR, present smaller scores (table 1). This might suggest A2M plays a role in androgen sensitive, AR positive prostate cancer progression.

Materials and Methods

Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017. Only proteins with Mascot scores of approximately 90 or >90 were considered. The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at https://zenodo.org/records/7653082#.Y_DxJXbP3IV

References:

1. Pedro A (2023).Hemoglobin in Prostae cancer Cancer (ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.30186.59842

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