A2M in Lung Cancer – short report (ISSN 2753-8176 (online))

A2M in Lung Cancer – short report (ISSN 2753-8176 (online))

1.Ana Pedro

1.Gwyntwr1386 Health & Social Care, 58 Grove Road, Birkenhead, CH42 XX. info@gwyntwr1386.com

Alpha-2-macroglobulin (A2M) is a large protein found in the blood which is mainly produced by the liver. A2M is the largest major non-immunoglobulin protein in human plasma. Alpha-2-macroglobulin (A2M) is a large protein found in the blood which is mainly produced by the liver. A2M is the largest major non-immunoglobulin protein in human plasma.

We study the possibility that A2M can play a role in lung cancer initiation and/or progression.

Here we analyse by proteomic analysis, the presence of A2M (P010230) in the precipitates derived from conditioned media of different lung cancer cell lines and lung cancer-derived bone metastases (mets) (table 1). We find out that control cell WI38 presents quite a big Mascot score (993), while primary lung cancer cell lines present an average Mascot score level (315-415) and those derived from lymph node (LN) metastases present a slightly higher Mascot score range (330-455). Finally, the bone metastases sample presents a huge Mascot score (1766). This suggests that A2M may have a role in inhibiting lung cancer initiation and progression.

Materials and Methods

Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017. Only proteins with Mascot scores of approximately 90 or >90 were considered. The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at Hemoglobin in Prostate Cancer | Zenodo and https://zenodo.org/record/8130817 for additional files.1118 - lung cancer cell line, WI38 - 3 month old female embryo lung tissue cell line. Please, refer to materials and methods section at Pedro A (2023).Hemoglobin in Lung Cancer - Short report (ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.30186.59842

References:

1. Pedro A (2023).Hemoglobin in Lung Cancer - Short report (ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.30186.59842

2. NCI-H292 [H292] - CRL-1848 | ATCC

3.NCI-H1650 [H-1650, H1650] - CRL-5883 | ATCC

4.ECACC General Cell Collection: 90071810 PC-9 (formerly known as PC-14) (culturecollections.org.uk) , Cellosaurus cell line PC-9 (CVCL_B260)

5.NCI-H358 [H-358, H358] - CRL-5807 | ATCC

6.NCI-H1975 [H-1975, H1975] - CRL-5908 | ATCC

7.NCI-H2228 [H2228] - CRL-5935

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