A2M function in pancreatic cancer in mouse (ISSN 2753-8176 (online))

1.Ana Pedro

1.Gwyntwr1386 Health & Social Care, Harold Tomlin Day Centre, 24 Grosvenor Street, Chester, CH1 2DD. info@gwyntwr1386.com

Introduction, results and discussion

In our previous article we analyzed the hypothesis that A2M predicts cancer appearance or progression in the breast (1) and in the skin (2), now we analyse if this hypothesis might be true as well in the case of pancreatic cancer in mouse.

The most common type of pancreatic cancer (PC) is adenocarcinoma, which accounts for 90% of the cases. Around 1-2% of the PC cases have a neuroendocrine origin, arising from the hormone-producing cells of the pancreas being in general less aggressive than the adenocarcinoma (3). In table 1, we found Mascot scores for two different mouse normal pancreas tissue CM samples (one shows a significant Mascot Score, while the other shows no A2M), PanIn cell line CM samples (precursor to invasive ductal cell adenocarcinoma, precancerous), Pan02 (mouse adenocarcinoma cell line) CM samples and a plasma Pan02 sample. A2M is present in one of the PanIn CM samples with a huge Mascot score while in the other sample is not present. Finally, one of the Pan02 CM samples shows a non-significant (NS) A2M Mascot score while in the other there is no A2M present and the Pan02 plasma sample shows a massive Mascot Score.

Once again these results suggest that A2M might be involved not only in defining which normal tissues will develop to cancer but also in determining which cancers will progress further.

Methods

Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017 (4). Only proteins with Mascot scores of approximately 90 or >90 were considered (5). The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at https://zenodo.org/record/733946#.Y3pC5XbP3IU

References:

1. https://gwyntwrscience.blogspot.com/2025/07/does-alpha-2-macroglobulin-2m-predicts.html

2. Pedro A (2022). Hemoglobin in Pancreatic cancer - short report (ISSN 2753-8176 (online))

3. Pedro A (2025). Does A2M predicts cancer appearance or progression in skin? (ISSN 2753-8176 (online))

4. Hamidi et al. (2017) Identification of potential blood-derived extracellular vesicles biomarkers to diagnose and predict distant metastases in ER+ breast cancer patients, bioRxiv preprint doi: https://doi.org/10.1101/202291;

5. http://www.matrixscience.com/help/scoring_help.html