Gwyntwr1386 Science Journal

Insights into the transition between G2/M arrest and apoptosis triggering in HeLa cells DOI: 10.13140/RG.2.2.18707.66083, ISSN 2753-8176 (online) Ana Pedro 1 1 Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK info@gwyntwr1386.com MTC (2-Methoxy-5-(2’, 3’, 4’-trimethoxyphenyl)-2,4,6-cycloheptatrien-1-one) is a bicyclic colchine analogue synthesized by Fitzgerald (1) and induces microtubule disruption quickly and reversibly (2, 3) leading to a G2/M cell cycle arrest what culminates in apoptosis in human leukemic cells (Gajate et al, 2000) and in HeLa cells (4). B-cell lymphoma-extra large (Bcl-xL), is a member of the Bcl-2 family of proteins. It is a transmembrane molecule localized in the mitochonria and acts as an anti-apoptotic protein by preventing the release of mitochondrial contents such as cytocrome c, which leads to caspase activation and ultimately, apoptosis triggering (5). Here we analyse the events which occur during the tran...

The effects of MTC in the cell cycle, cytoskeleton and apoptosis pathways in HeLa cells Ana Pedro 1 1 Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK info@gwyntwr1386.com MTC (2-Methoxy-5-(2’, 3’, 4’-trimethoxyphenyl)-2,4,6-cycloheptatrien-1-one) is a bicyclic colchine analogue synthesized by Fitzgerald (1) and induces microtubule disruption quickly and reversibly (2, 3) leading to a G2/M cell cycle arrest what culminates in apoptosis in human leukemic cells (Gajate et al, 2000). HeLa is the oldest human cell line which is derived from cervical cancer cells taken on 1951, from Henrietta Lacks, a 31-year-old African-American (4). Here we analyse the action of MTC in HeLa cells. Time-course of MTC in Hela cells In the present study our goal was to characterize the action of MTC on solid tumor cell lines such as HeLa. To this aim, HeLa cells were introduced with MTC (1μm) during different treatment hours. We found that HeLa c...

THE ROLE OF HEMOGLOBIN IN CANCER – AN OPINION ISSN 2753-8176 (online), DOI:10.13140/RG.2.2.17696.97281 Ana Pedro1 1Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK We have identified by proteomics analysis a possible biomarker for early stage ER+ breast cancer (BC), HCG1745306 isoform CRA-a, an isoform of haemoglobin subunit alpha (HBA) (1). A large body of evidence supports a role for haemoglobin not only in BC diagnosis but also in cancer diagnosis in general. HBA has also been previously found to be upregulated in the urine of patients with early invasive BC (2). Also, previous work indicated the possibility of a direct relationship between the hemoglobin level and not only the early stages of cancer, but also the chemically induced precancerous condition (3). Total haemoglobin (THC) has been shown to be present in higher concentrations in breast tumours in comparison to healthy tissues while oxyhemoglobin has been showed to be reduce...

A Review of The Role of Hemoglobin in Different types of Cancer ISSN 2753-8176 (online), DOI:10.13140/RG.2.2.24610.20164 Ana Pedro1 1Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK Keywords: 1. Corresponding author: info@gwyntwr1386.com A role for haemoglobin in cancer diagnosis in general is supported by a good number of publications. A previous work indicated the possibility of a direct relationship between the hemoglobin level and not only the early stages of cancer, but also the chemically induced precancerous condition (1). Interestingly, in patients with a diagnosis of primary lung, colorectal, breast or liver cancer, higher post-diagnosis haemoglobin change regardless of the baseline haemoglobin levels and the direction of changes associates with a significantly shorter survival (2). Here we summarize, the results of our findings in what concerns proteomics analyzis of several different cancer patient samples and of cancer cell line...

Therapy with DNA damaging drugs of a small number of non-metastatic and metastatic colorectal cancer patients Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Introduction and results Colorectal cancer (CRC) is the third most common malignancy and the second most deadly cancer, inducing estimated 1.9 million incidence cases and 0.9 million deaths worldwide in 2020 (1). Each year, approximately, 230,000 patients are treated with adjuvant chemotherapy (AT) (2) . Nine patients with non-metastatic (NM) and metastatic (M) CRC were treated with 5-fluorouracil (5-FU)/leucovorin (LV) (LV5FU) regimen (3), 3 patients with M disease with FOLFOX (folinic acid (leucovorin, FOL), fluorouracil (5-FU, F), and oxaliplatin (Eloxatin, OX) (4) and 2 patients with NM and M disease were treated with XELOX regimen (capecitabine (trade name Xeloda) combined with oxaliplatin (5) (tables 1 and 2). All the patients...

Hemoglobin in Acute Leukemias, Brief Report Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Introduction Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells, where there is a rapid growth of abnormal cells that build up in the bone marrow and blood interfering with normal blood cell production (1). Here, we analyze by proteomic analysis the precipitates after ultra-centrifugation of the conditioned media resulting from the cell culture of OCI-AML3 and Kasumi1 cell lines. We compare these proteomic profiles with those derived from the precipitates after ultra-centrifugation of plasma samples of control adult males (Control plasma male 1 and Control plasma male 2) OCI-AML3 cell line is derived from a 57 year-old male with Adult acute myeloid leukemia (2). Kasumi1 cell line is derived from the peripheral blood of a male AML patient, 7 weeks old (3). Resul...

Hemoglobin and Children’s cancers - Brief Report, DOI:10.13140/RG.2.2.21321.52325, ISSN 2753-8176 (online) Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom info@gwyntwr1386.com Wilms tumor or nephroblastoma , is a cancer of the kidneys which occurs most commonly as a renal tumor in child patients (1). Neuroblastoma, a rare type of cancer, also mostly affects babies and young children. It develops from neuroblasts which were left behind during baby's development in the womb (2).Most often starts from one of the adrenal glands but can also develop in the neck, chest, abdomen or spine (3). IMR5 is a neuroblastoma cell line, derived from a male, aged 1 year and 1 month, derived from a metastatic site in the abdomen (4). SKNBe2 is another neuroblastoma cell line, derived from a 2 year old male and from bone marrow metastasis after chemotherapy and radiotherapy (5). Finally, CCG9911 is a Wilm’s tumor cell line (6)...