A2M IN BONE CANCER (ISSN 2753-8176 (online)) - brief report

A2M IN BONE CANCER (ISSN 2753-8176 (online)) - brief report

1.Ana Pedro

1.Gwyntwr1386 Health & Social Care, 58 Grove Rd, Birkenhead, CH42 3XX. info@gwyntwr1386.com

Osteosarcoma is a type of bone sarcoma or primary bone cancer which usually develops in growing bones and can affect any bone although most common areas are the arms or legs. It is most often found in teenagers and young adults, although it can happen at any age. Around 1 in 10 osteosarcomas (10%) develop in adults in their 60s and 70s (1). Ewing tumors, however can start in either the bone or the soft tissue (2). Here, we analyzed by proteomics analysis, the content of the precipitates derived from the ultracentrifugation of conditioned media derived from the cell culture of different bone cancer cell lines (table 1). We found out that A2M is present in all cancer cell lines types with significant scores and that is present in higher levels in the osteosarcoma cell line 143B rather than in Ewing’s sarcoma cell line SKES1 what suggests that A2M has a more relecant role is osteosarcoma progression rather that in Ewing’s sarcoma progression.

Materials and Methods

Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017 (8). Only proteins with Mascot scores of approximately 90 or >90 were considered (9) The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at Hemoglobin in Head and Neck cancer - Short report (ISSN 2753-8176 (online) | Zenodo

References 1. Osteosarcoma | Macmillan Cancer Support 2. Soft tissue sarcoma | Macmillan Cancer Support 3. SK-ES-1 - HTB-86 | ATCC 4. Saos-2 - HTB-85 | ATCC 5. U-2 OS - HTB-96 | ATCC 6. 143B - CRL-8303 | ATCC 7. PAUTKE et al. (2004). Characterization of Osteosarcoma Cell Lines MG-63, Saos-2 and U-2 OS in Comparison to Human Osteoblasts. ANTICANCER RESEARCH 24: 3743-3748 8. https://www.biorxiv.org/content/10.1101/202291v1 9.https://gwyntwrscience.blogspot.com/2023/05/hemoglobin-and-osteoblasts-markers-in.html. 10.https://f1000research.com/articles/7-283

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