Hemoglobin in Pancreatic Cancer – short report, ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.20722.79043

Hemoglobin in Pancreatic Cancer – short report, ISSN 2753-8176 (online), DOI: 10.13140/RG.2.2.20722.79043 Ana Pedro (1) 1. Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway,Chester CH4 9QR, United Kingdom anapedrolaboratories@gmail.com Some cancer cell types such as from prostate, lung and breast cancers might express hemoglobin beta , which might be a defense mechanism of tumor cells against intracellular reactive oxygen species (ROS) (1). We examine the presence of hemoglobin subunits in different types of pancreatic adenocarcinoma cell lines cell culture conditioned media (CM)(https://zenodo.org/record/7339463#.Y3pC5XbP3IU). The most common of pancreatic cancer (PC) is adenocarcinoma, accounting for about 90% of cases. Around 1–2% of cases of PC have a neuroendocrine origin , arising from the hormone-producing cells of the pancreas. These tumours in general are less aggressive than pancreatic adenocarcinoma. PanIN (pancreatic intraepithelial neoplasia, a histologically well-defined precursor to invasive ductal adenocarcinoma of the pancreas ) cell line was isolated from Pdx-1-Cre; LSL-KrasG12D/+ mouse and it was shown KrasG12D induces ductal lesion PanINs (2). Pan02 is a well-established Grade III adenocarcinoma model developed by chemical induction with 3-MCA (3-methylcholanthrene) in male C57BL/6 mice (3). We found out that normal mouse pancreas tissue CM does not show any hemoglobin subtype (Fig.1). However, PanIn CM may show good amount of hemoglobin subunit beta and vestigial amounts of hemoglobin subunit alpha. By other side, AsPc1 CM shows good amounts of both types of hemoglobin subunits (fig.1). The remaining samples only show hemoglobin subunit alpha: BXPC3 CM shows vestigial amounts, HPAF2 CM vestigial amounts or a good amount, MiaPaca2 CM a good amount, PAN02 CM a good amount or nothing and PANC1 CM nothing. These results suggest that in PC, similarly to what might happen with other cancer types (1), hemoglobin beta might play a role, in particular in the initial stages of PC initiation. Hemoglobin alpha might have a role in established PC. Methods Proteomic analysis was performed at the Rockefeller University, Proteomics Center as described in Hamidi et al., 2017 (4). Only proteins with Mascot scores of approximately 90 or >90 were considered (5). The proteomic analysis excel files and each correspondent sample details were generously and kindly shared and donated by Dr. David Lyden, Weill Cornell Medical College, New York, USA. Original data files can be found at https://zenodo.org/record/7339463#.Y3pC5XbP3IU References 1. Zheng et al (2017). Expression of β-globin by cancer cells promotes cell survival during blood-borne dissemination. Nature Communications 8, Article number: 14344 2. Shen et al (2013). The biological features of PanIN initiated from oncogenic Kras mutation in genetically engineered mouse models. Cancer Lett,doi: 10.1016/j.canlet.2013.07.010. 3. Corbett et al (2018). Combination immunotherapy and radiation therapy strategies for pancreatic cancer – Targeting multiple steps in the cancer immunity cycle. J Gastro Oncol,9(6):1014-1026 4. Hamidi et al. (2017) Identification of potential blood-derived extracellular vesicles biomarkers to diagnose and predict distant metastases in ER+ breast cancer patients, bioRxiv preprint doi: https://doi.org/10.1101/202291;5. http://www.matrixscience.com/help/scoring_help.html

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Please, see tumor proteins only (mouse pancreatic cancer plasma) and control proteins only (mouse plasma) files at https://zenodo.org/record/8130724. Control proteins only sample contains no hemoglobins and tumor proteins only sample contains hemoglobin beta (P02088) with a Mascot Score of 208.62.