Gwyntwr1386 Science Journal

Insights into the transition between G2/M arrest and apoptosis triggering in HeLa cells DOI: 10.13140/RG.2.2.18707.66083, ISSN 2753-8176 (online) Ana Pedro 1 1 Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK info@gwyntwr1386.com MTC (2-Methoxy-5-(2’, 3’, 4’-trimethoxyphenyl)-2,4,6-cycloheptatrien-1-one) is a bicyclic colchine analogue synthesized by Fitzgerald (1) and induces microtubule disruption quickly and reversibly (2, 3) leading to a G2/M cell cycle arrest what culminates in apoptosis in human leukemic cells (Gajate et al, 2000) and in HeLa cells (4). B-cell lymphoma-extra large (Bcl-xL), is a member of the Bcl-2 family of proteins. It is a transmembrane molecule localized in the mitochonria and acts as an anti-apoptotic protein by preventing the release of mitochondrial contents such as cytocrome c, which leads to caspase activation and ultimately, apoptosis triggering (5). Here we analyse the events which occur during the tran...

The effects of MTC in the cell cycle, cytoskeleton and apoptosis pathways in HeLa cells Ana Pedro 1 1 Gwyntwr1386 Pharmacy, Regus Chester Business Park, Heronsway, Chester, CH49QR, UK info@gwyntwr1386.com MTC (2-Methoxy-5-(2’, 3’, 4’-trimethoxyphenyl)-2,4,6-cycloheptatrien-1-one) is a bicyclic colchine analogue synthesized by Fitzgerald (1) and induces microtubule disruption quickly and reversibly (2, 3) leading to a G2/M cell cycle arrest what culminates in apoptosis in human leukemic cells (Gajate et al, 2000). HeLa is the oldest human cell line which is derived from cervical cancer cells taken on 1951, from Henrietta Lacks, a 31-year-old African-American (4). Here we analyse the action of MTC in HeLa cells. Time-course of MTC in Hela cells In the present study our goal was to characterize the action of MTC on solid tumor cell lines such as HeLa. To this aim, HeLa cells were introduced with MTC (1μm) during different treatment hours. We found that HeLa c...